Zhuling

This product is Polyporus umbellatus(Pers.)Dry sclerotia of fries.Excavate in spring and autumn to remove sediment and dry.
[properties]this product is strip-shaped,quasi round or flat block,and some have branches,with a length of 5~25cm and a diameter of 2~6cm.The surface is black,grayish black or brownish black,with shrinkage or tumor like protrusion.The body is light and hard,and the section is almost white or yellowish white,slightly granular.The air is slight and the taste is light.
[identification](1)cut surface of this product:all are closely intertwined by hyphae.The outer layer is 27~54 thickμm.The hyphae are brown and difficult to separate;The internal hyphae are colorless,curved and 2~10 in diameterμm.Some can see transverse septum,with branches or nodular expansion.There are many calcium oxalate square crystals among the hyphae,mostly in the shape of square octahedron,regular biconical octahedron or irregular polyhedron,with a diameter of 3~60μm.Up to 68μm.Sometimes several crystal sets.
(2)Take 1g of this product powder,add 20ml of methanol,ultrasonic treatment for 30 minutes,filter,and take the filtrate as the test solution.Take ergosterol reference substance and add methanol to make a solution containing 1mg per 1ml as the reference solution.Test according to thin-layer chromatography(general rule 0502)and absorb 20%of the test solutionμl.Reference solution 4μl.Point on the same silica gel G thin layer plate respectively,use petroleum ether(60~90℃)-ethyl acetate(3∶1)as the developing agent,develop,take out,dry,spray with 2%vanillin sulfuric acid solution,and heat at 105℃until the spots are clear.In the chromatogram of the test sample,there are spots of the same color at the position corresponding to the chromatogram of the control sample.
[check]moisture Not more than 14.0%(the second method of general rule 0832).
Total ash Not more than 12.0%(general rule 2302).
Acid insoluble ash Not more than 5.0%(general rule 2302).
[content determination]determine according to high performance liquid chromatography(general rule 0512).
Chromatographic conditions and system suitability test Octadecyl silane bonded silica gel was used as filler;Methanol as mobile phase;The detection wavelength was 283nm.The number of theoretical plates shall not be less than 5000 according to ergosterol peak.
Preparation of reference solution Take an appropriate amount of ergosterol reference substance,accurately weigh it,and add methanol to make it,containing 50%per 1mlμG solution.
Preparation of test solution Take about 0.5g of this product powder(passing No.4 sieve),accurately weigh it,place it in a conical flask with a stopper,accurately add 10ml of methanol,weigh it,conduct ultrasonic treatment(power 220W,frequency 50KHz)for 1 hour,cool it,weigh it again,make up the lost weight with methanol,shake it well,filter it,and take the filtrate.
The determination method accurately absorbs 20%of the control solution and 20%of the test solutionμl.Inject it into the liquid chromatograph for determination.
This product is calculated as dry product,and the content of ergosterol(c28h44o)shall not be less than 0.070%.
Decoction pieces
[processing]remove impurities,soak,wash,moisten,cut thick pieces and dry.
[properties]this product is a kind of round or irregular thick piece.The outer skin is black or brownish black and wrinkled.The section is almost white or yellowish white,slightly granular.The air is slight and the taste is light.
[check]moisture No more than 13.0%of the same medicinal material.
The total ash content is the same as that of medicinal materials,and shall not exceed 10.0%.
[content determination]the content of ergosterol(c28h44o)in the same medicinal material shall not be less than 0.050%.
[identification](except the cut surface)[inspection](acid insoluble ash)is the same as that of medicinal materials.
[nature,taste and meridian returning]sweet,light and flat.Return to kidney and bladder meridian.
【functions and indications】promoting water and dampness.It is used for adverse urination,edema,diarrhea,drenching turbidity and subduction.
[usage and dosage]6~12g.
[storage]place in a ventilated and dry place.