Zhebeimu

This product is Fritillaria thunbergii Miq Dry bulb.When the plants wither in early summer,dig and wash them.The size is separated,and the larger one removes the core bud,which is commonly known as"scallop";Small people do not go to the core bud,which is often called"pearl shell".Rub separately,remove the skin,mix with calcined shell powder,absorb the wiped slurry and dry;Or take the bulb,separate the size,wash,remove the core bud,cut it into thick slices while fresh,wash and dry,which is commonly known as"Zhejiang shellfish slices".
[characters]the scallop is a single leaf in the outer layer of the bulb,slightly crescent shaped,1~2cm high and 2~3.5cm in diameter.The outer surface is off white to light yellow,and the inner surface is white or light brown,covered with white powder.Hard and brittle,easy to break,white to yellowish white section,rich in powder.It tastes slightly bitter.
Pearl shell is a complete bulb,which is oblate,1~1.5cm high and 1~2.5cm in diameter.The surface is yellowish brown to yellowish brown,with irregular wrinkles;Or the surface is off white to light yellow,smooth or covered with white powder.Hard,not easy to break,light yellow or white section,slightly horny or powdery;The outer scale leaves are 2-valved,plump,slightly kidney shaped,embracing each other,and there are 2-3 small scale leaves and dry shrunk residual stems.
Fritillaria thunbergii tablets are oval or quasi circular,with different sizes,1.5~3.5cm long,1~2cm wide and 0.2~0.4cm thick.The outer skin is yellowish brown or grayish brown,slightly wrinkled;Or light yellow,smooth.The section is slightly bulging and grayish white;Or flat,pink white.It is brittle and easy to break.The flour is white and rich in powder.
[identification](1)the powder of this product is yellowish white.There are many starch grains,single oval,wide oval or oval,with a diameter of 6~56μm.The lamination is not obvious.Epidermal cells are polygonal or rectangular,and the vertical peripheral wall is Beaded thickened;Stomata are rare,with 4~5 accessory guard cells.Calcium oxalate crystals are rare,small,mostly granular,and some are spindle,square or fine rod-shaped.The conduit is mostly threaded,with a diameter of up to 18μm。(2)Take 5g of this product powder,add 2ml of concentrated ammonia test solution and 20ml of chloroform,place it overnight,filter it,take 8ml of filtrate,evaporate it to dryness,and add 1ml of chloroform to dissolve the residue as the test solution.In addition,take the reference substance of Beimu A and Beimu B and add chloroform to make a mixed solution containing 2mg per 1ml as the reference solution.Test according to thin-layer chromatography(general rule 0502),and absorb 10~20 test solutionμl.Reference solution 10μl.Point on the same silica gel G thin-layer plate respectively,use ethyl acetate methanol concentrated ammonia test solution(17:2:1)as the developing agent,develop,take out,dry,and spray dilute bismuth potassium iodide test solution.In the chromatogram of the test sample,there are spots of the same color at the position corresponding to the chromatogram of the control sample.
[inspection]the moisture content shall not exceed 18.0%(the second method of general rule 0832).The total ash content shall not exceed 2300.2%.
[extract]according to the hot leaching method under the alcohol soluble extract determination method(general rule 2201),dilute ethanol shall be used as the solvent,which shall not be less than 8.0%.
[content determination]determine according to high performance liquid chromatography(general rule 0512).
The chromatographic conditions and system suitability test take octadecyl silane bonded silica gel as filler;Acetonitrile water diethylamine(70∶30∶0.03)was used as mobile phase;Evaporative light scattering detector.The number of theoretical plates shall not be less than 2000 according to the peak of Beimu a.Preparation of reference solution take a proper amount of Beimu a reference and Beimu B reference,weigh accurately,and add methanol to make a mixed solution containing 0.2mg of Beimu A and 0.15mg of Beimu B per 1ml.
Preparation of the test solution:take about 2G of the powder(passing No.4 sieve)of the product,accurately weigh it,put it into a flask,add 4ml of concentrated ammonia test solution to soak for 1 hour,accurately add 40ml of chloroform methanol(4:1)mixed solution,weigh it,mix it evenly,heat it in an 80℃water bath for reflux for 2 hours,cool it,weigh it again,add the above mixed solution to make up the lost weight and filter it.Accurately measure 10ml of continuous filtrate,evaporate it in an evaporating dish,add methanol to dissolve the residue and transfer it to a 2ml measuring bottle,add methanol to the scale and shake well.
The determination method accurately absorbs 10%of the reference solution respectivelyμl、20μl.Test solution 5~15μl.Inject it into the liquid chromatograph for determination,and calculate the contents of Beimu A and Beimu B respectively with the logarithm equation of external standard two-point method.
This product is calculated as a dry product,and the total amount of Beimu a(c27h45no3)and Beimu B(c27h43no3)shall not be less than 0.080%.
[processing]remove impurities.If not sliced,wash,moisten thoroughly,cut into thick pieces and dry;Or break into pieces.
【properties】Fritillaria thunbergii is a kind of thick round pieces or fragments,and some have heart buds.The outer skin is yellowish brown or grayish brown,slightly wrinkled;Or yellowish white,smooth or covered with white powder.The section is slightly bulging or flat,gray white or pink white,slightly horny or rich in powder.It is hard and easy to break;Or hard,the section is gray white or white,and some are light yellowish brown.It has a slight breath and a bitter taste.
[identification][inspection][extract][content determination]is the same as that of medicinal materials.
[nature,taste and meridian returning]bitter,cold.Return to lung and heart meridian.
[functions and indications]clearing heat,resolving phlegm,relieving cough,detoxifying,dispersing knot and eliminating carbuncle.It is used for wind heat cough,phlegm fire cough,lung carbuncle,breast carbuncle,scrofula and sore poison.
[usage and dosage]5~10g.
[note]it should not be used with Radix Aconiti,Radix Aconiti,Radix Aconiti and aconite.
[storage]store in a dry place to prevent moths.